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A tBLASTn analysis (E-value cutoff = 1e-5) using these hypothetical proteins as queries was conducted against the T. urticae genome (http://bioinformatics.psb.ugent.be/orcae/overview/Tetur). Gene models were refined or created on the basis of homology, RNA-seq and/or EST support and RT-PCR.
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Niederhofer says about 50,000 people accessed the site in April – but over 30 million queries were conducted via the API, many of these from customer sites that pay for the API.
These queries are conducted to extract all peaks from two subspaces of types L and H with lower and higher m/z repeatedly centered at each LC-HRMS peak.
To extend the database search, tblastn queries were conducted against the nucleotide nr and EST-others databases at GenBank.
Genome queries were conducted by direct searches of the most recent Drosophila genome (BDGP 4.3) using UNIX grep and perl.
Queries are conducted by stepwise entry of input criteria with each query imposed on the previous so that all genes currently displayed meet all preceding query criteria as well as the criterion that was last entered.
For purpose of this study, three separate queries were conducted, one using the programs duplicate result filter (duplicate-free method – DFM), the other without any data correction (raw data method – RDM).
Queries were conducted using the following command structure: blat -q=prot -t=dnax -minIdentity=70 –extendThroughN [genome file] [query file] [output file] blat -q=prot -t=dnax -minIdentity=70 –extendThroughN [genome file] [query file] [output file] In the case of MultiZ (Blanchette et al. 2004), CCDS orthologs were downloaded directly from the UCSC genome browser (Kent et al. 2002).
The initial query was conducted through the i2b2 workbench by our clinical research team, which produced a de-identified dataset.
An initial literature query was conducted in the following four reference databases using search strategies as summarized in Table 1.
The present query was conducted in order to examine the intervention's effect on QOL outcomes, depression, self-efficacy and eating behavior, possible mediators of behavior change.
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