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No positive effect of buffering or increasing the ionic strength of the quenching solution was observed.
In the rapid cold quenching area, the cold quenching solution is delivered at the quenching solution inlet, to be mixed with the nanoparticle colloids at the Y mixer 2, following which the mix flows through the quenching channel.
For a quenching solution of (1.1), the inf norm of one of the components must tend to as tends to the quenching time.
The surface of the NPs can be rapidly deactivated through the formation of an ultra-thin oxidation layer caused by including acetone in the quenching solution.
The reactor consists of three regions: the mixing and reaction area from the inlets of 1 and 2 to Y mixers connected with channels shrinking from 200 to 30 μm, the nucleation and growth area with channel width of 60 μm and length of ~30 cm, and the rapid cold quenching area with the cold quenching solution delivered at the quenching solution inlet.
The broth was directly injected into a tube with 15 mL pre-cooled quenching solution (60% methanol, −40 °C) (Carnicer et al. 2012) and then be filtered by a cellulose acetate fiber film (Millipore, USA).
To investigate a possible quenching solution of problem (1), it is advantageous to convert the initial-boundary problem (1) into an associated integral equation by the technique in [7].
Quenching solution was then removed and cell lysates were prepared as described below.
Quenching solution D had the smallest relative standard deviation for replicate samples.
Interestingly, using quenching solution A gave higher intra- and extracellular concentrations of fumarate.
For mass spectrometry studies, further consideration should be given to any buffer additives to the quenching solution.
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