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Thermal quenching ratio.
The thermal quenching ratio (R Q) is a key parameter to evaluate the affection of temperature on luminescence quenching [16].
The quenching ratio parameter QR is introduced to consider the quenching of the flame by the piston.
The effects of doping Tm3+ ions on thermal quenching ratio, population mechanism of thermally coupled levels, and temperature sensitivity are also observed by using the different excitation powers.
It was found that the spectrum structure, thermal quenching ratio, fluorescence intensity ratio, and sensitivity from thermally coupled levels were strongly dependent on the change of pump powers.
The spectrum, thermal quenching ratio, fluorescence intensity ratios, and optical temperature sensitivity of the transparent glass ceramics are observed to be dependent on the pump power.
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The resulting quenching ratios (FO/ F) as well as the lifetime quenching ratios (⟨τO⟩/⟨τ⟩) are shown in Figures 5 and 6 of the Supporting Information; both ratios show a slight increase with temperature for both Trp and Tyr (∼1 2% per degree Celsius).
Fig. 6 Thermal quenching ratios (RQ) of (a) NGF1, (b) NGF3 at low 66.8 mW/cm2 excitation power and at high 322.4 mW/cm2 excitation power.
We next used the combination of intensity and lifetime quenching ratios to calculate the relative amount of static quenching, dynamic quenching, and unquenched fluorescence components.
For both Tyr- and Trp-containing samples, both the intensity (FO/ F) and the lifetime (⟨τO⟩/⟨τ⟩) quenching ratios were measured, where an O subscript indicates data from the unquenched, Phe-containing sample.
However, even with this contact, bimane quenching by Phe is minimal, as indicated in the comparison of intensity quenching ratios, calculated using N116/N132B as the unquenched control, 1.3 for the Phe-containing sample (N116F/N132B) versus ∼14.0 for the Tyr-containing sample (N116Y/N132B) and ∼27.0 for the Trp-containing sample (N116W/N132B).
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