Sentence examples for quench flow from inspiring English sources

At each specific time point, equal volumes of 0.4 μM ternary complex and 2 μM programmed ribosomes were mixed and quenched with 40% formic acid using KinTek quench flow instrument at 20 °C.

Rapid quench flow techniques thus provide a powerful method for studying rapid reactions that occur in the millisecond time frame.

In this chapter, we describe experimental design and procedures for kinetic characterization of the yeast SLN1 YPD1 SSK1 osmoregulatory phosphorelay system using a rapid quench flow kinetic instrument.

Reactions were carried in enzyme excess by rapid quench analysis [42] for WT using a KinTek RQF-3 Rapid Quench Flow apparatus, and manually for the D211N mutant.

AB carried out Quench Flow experiments.

Quench-flow experiments were performed using the RQF-63 Hi-Tech Rapid Quench Flow system (TgK Scientific) to elucidate the extent and kinetics of nicking of various DNA constructs by RepD.

(E ) Fluorescent images of nonreducing SDS-PAGE gels showing rapid-quench flow pulse-chase fluorescent Ub transfer from E2 to Rsp5WW3-HECT to Sna3C, for the indicated versions of Rsp5WW3-HECT.

The behavior of the mutants in quench-flow experiments to determine the folding pathway was exactly as predicted by the changes in the buried surface area parameter calculated from the amino acid sequence.

The chapter also addresses eukaryotic initiation factor purification, nuclear magnetic resonance double-stranded RNA-activated protein kinase complexes, quench-flow time-resolved chemical probing of ribosomeligand interactions, rabbit reticulocyte lysate, and sedimentation velocity analytical ultracentrifugation.

The reaction was initiated by rapid mixing of the IC and TC in a quench-flow instrument.

The amplitude of the quench-flow transient shows ∼80% of the DNA is nicked (Table 1).