Lack of EC activity at higher temperatures was consistent with dehydration and quenching of FeII states accompanied with change of color from blue to rust (FeIII) typical of Fe2O3.
The dehydration may account for fluorescence quenching, as fluorescein and DCF in the gas phase are essentially nonfluorescent.
When people urinate, they can become thirsty, and if they drink more alcohol to quench this thirst, a cycle begins, leading to dehydration.
In addition, the dehydration will promote thirst that is quenched by drinking well water that is often contaminated with heavy metals and pesticides [ 24].
The previously prepared slide was denatured in 70% formamide/2 × SSC, at 65°C for 80 sec, and quenched in an ice-cold 70% ethanol followed by dehydration in 70%, 90%, and 100% ethanol series at room temperature.
Due to the fact that when employing wet chemical methods the ZnO crystallites are formed by Zn(OH 2 dehydration, traces of this compound on the ZnO surface lead to the quenching of the ZnO exciton emission [40].
After deparaffinization and dehydration, microwave antigen retrieval was performed for 5 min, followed by peroxidase quenching with 0.6%H2O2O2 in phosphate-buffered saline (PBS) for 15 min.
After deparaffinization and dehydration, microwave antigen retrieval was performed for 5 min prior to peroxidase quenching with 3%H2O22 in phosphate buffered saline (PBS) for 15 min.
After deparaffinization and dehydration, microwave antigen retrieval was performed for 5 min prior to peroxidase quenching with 3%H2O22 in PBS for 15 min. Subsequently, sections were preblocked with 5% bovine serum albumin for 30 min and incubated with a primary antibody (anti-CD68) overnight at 4°C.
After a standard dehydration-rehydration procedure, liver sections were incubated with 3%H2O22 for 10 min to quench endogenous peroxidase activity.
