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RNA quality and quantity were determined using a Nanodrop 2000 spectrophotometer (Thermo Fisher Scientific).
RNA quality and quantity were determined by photometry.
MtDNA levels as a marker of mitochondrial quantity were determined by PCR.
RNA quality and quantity were determined by gel electrophoresis and photometry.
The dye-incorporation ratio and the cRNA quantity were determined using the Nanodrop spectrophotometer.
Between each purification step, RNA quality and quantity were determined by Bioanalyzer (Agilent) using the RNA Nano chips, and quantified using the ND-8000 (NanoDrop Technologies).
RNA was isolated using the RNeasy mini kit (Qiagen) and quality (260/280 nm ratio) and quantity were determined using a Nanodrop® ND-1000 system (Witec AG, Switzerland).
The quality and quantity were determined by nanodrop UV spectroscopy and analyzed on a RNA 6000 Nano Labchip using 2100 bioanalyzer (Agilent technologies).
Total RNA was isolated from control and hUCBSC-treated mice brains using Rneasy kit (Qiagen, Valencia, CA) and quantity were determined by A260 measurement.
Nucleic acid quality and quantity were determined as described previously.
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cDNA quantity was determined with the Qubit Flourometer (Life Technologies, Grand Island, NY) and quality assessed using the Agilent Bioanalyzer 2100 (Santa Clara, CA).
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