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Confidence limits for this quantity were calculated using Wilson's method for Binomial data [ 27].
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The relative quantity was calculated using the equation RQ = 2−ΔΔCT, where RQ is the relative quantity of the target gene.
Relative quantity was calculated using the ΔΔCt method.
Relative fold change in gene quantity was calculated using naïve resistant mice as a reference.
GAPDH was used as an endogenous control and the gene expression relative quantity was calculated using the ΔΔCt method.
Mean velocity vector field, corresponding vorticity contours, streamline topologies and turbulent quantities were calculated using 300 instantaneous velocity vector field measured by PIV.
Relative transcript quantities were calculated using the ΔΔCt method.
A set of results of these integrated quantities were calculated using the Fortran code for ≃150 different values of q between 0.01 and 100, nearly equally spaced in (log q)).
Relative transcript quantities were calculated using the ΔΔCt method with β-actin as an endogenous control for normalization.
Specific proteins relative quantities were calculated using the Image J software (NIH) with subsequent averaging of three separate experiments.
Absolute quantities were calculated using standard curves.
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