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Determination of protein quantity was performed in each well (cell lysate) for which supernatant was quantified for cytokines.
Normalization for cDNA quantity was performed for each template with an Elongation factor gene (At5g60390) as control gene using the relative standard curve method (delta CT) according to the Biorad instructions.
Signal noise was reduced by digital filtering process and conversion from voltage to defined physical quantity was performed and the bioscope allows instant signal monitoring, synchronized recording, and assisted sensor calibration.
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Sensitivity analysis excluding the study requiring the use of imputed quantities was performed; results were stable in this analysis.
Statistical evaluation of differences in protein quantities was performed using analysis of variance with genotype and viral infection included as the factors.
Normalization of soluble scFv quantities was performed by coating ELISA plate with 2 μg/mL of a monoclonal anti-poly Histidine antibody (H1029, Sigma).
Matching of quantities is performed in the space of characteristic variables as suggested by Kopriva [Appl. Numer. Math. 2 (1986) 221; J. Comput. Phys. 125 (1996) 244].
Quality and quantity check was performed by using Agilent 2100 bioanalyzer.
Comparison of quantity indicators was performed using ANOVA module.
RNA quality and quantity control was performed using NanoDrop ND1000 and BioAnalyzer (Agilent Technologies).
Quantity assessment was performed by visualising a box plot of the normalised data.
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CEO of Professional Science Editing for Scientists @ prosciediting.com