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Double digests were performed in the most suitable buffer, and the quantity of the least active enzyme per reaction was doubled if required.
In brief, we used QPCR to detect and quantify Enterococcus and Bacteroides in water samples based on the collection of these organisms on membrane filters, extraction of their total DNA, and PCR amplification (i.e., a process whereby the quantity of DNA is doubled in each cycle of amplification) of a genus-specific DNA sequence using the TaqMan PCR product detection system.
These Internet-era technologies, by one estimate, are doubling the quantity of business data every 1.2 years.
We would favor the rigorous analysis and curating of knowledge available in the scientific literature, which is doubling in quantity every decade since the 1950s.
We live in a world of big data, where the quantity of information generated by companies is doubling almost every year.
JASMIN — a facility that is part supercomputer, part data centre — is doubling its storage capacity, allowing scientists to access vast quantities of data and perform their own analyses.
Glioma cell lines were transfected with recombinant retroviral vectors using a modified Lipofectamine™ 2000 protocol (Invitrogen, California, U.S ., in which the quantity of plasmid DNA was double of the recommended quantity in order to achieve the highest transfection efficiency.
The quantity was good.
The quantity was not disclosed.
RNA quantity was determined photospectrometrically.
RNA quantity was evaluated spectrophotometrically.
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CEO of Professional Science Editing for Scientists @ prosciediting.com