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The appropriate protein quantity was dissolved in Laemli buffer (Tris HCl pH 6.8 62.5mM, glycerol 10%, SDS 1%, 2-mercapto ethanol 5%, bromphenol blue 0.0025%) and the proteins were separated in SDS-PAGE gels (12%) before they were blotted onto Nitrocellulose Transfer membrane (Whatman - Protrans).
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The test quantity of protein was dissolved in sterile water.
A 4.307 g quantity of anhydrous piperazine was dissolved in 20 ml PIPES solution to which 6.25 ml of 12 M HCl was carefully added.
A 10.6 nmol quantity of lyophilized 2 was dissolved in a 0.25 M HCl/25%MeOH/75%% H2O solution in a 600 μL Eppendorf tube and left to react at 40 °C for 1 h.
In this way, to operate near isothermal conditions and to minimize catalyst deactivation effects, a known quantity of methanol (10 ml) was dissolved into the isooctane as the solvent (250 ml) and the resulting mixture was charged into the reactor along with a predetermined quantity of catalyst (3 g).
Calculated quantity of the methanolic extract was dissolved in methanol in order to get 500 μg concentrations.
Briefly, weighed quantity of drug (50 mg) was dissolved in different combinations of oil, surfactant, and cosurfactant (1 mL).
Few quantity of the each portion was dissolved in water and filtered; to this 2 mL of the 10% aqueous sodium hydroxide was later added to produce a yellow colouration.
An accurately weighed quantity equivalent to 20 mg ETD was dissolved in 20 mL of methanol and transferred to a 100 mL calibrated flask.
The residue was dissolved in known quantity of methanol and transferred into a 250-ml calibrated flask and made up to the mark.
The accurately weighed amounts of phospholipid, surfactant, cholesterol, and drug were taken in a clean, dry, round-bottom flask, and this lipid mixture was dissolved in small quantity of chloroform-methanol mixture (3 : 1).
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com