Pearson's correlation analysis was used to estimate relative degree by quantity of the expression of the three genes.
To screen quantity of the expression of FBPase isozymes in KLN-205 cells, mouse FBP1-specific primers (forward 5′-CAGCTGCTGAATTCGCTCTG, reverse 5′-ACATTGGTTGAGCCAGCGATA) and mouse FBP2-specific primers (forward 5′-GACCCTCTGGATGGATCTTCA, reverse 5′-CAGAAGGCTCATCCTCCGT) were used.
Using only the data of genes represented on both microarray platforms (n = 1,271), we applied a methodology evaluated by Warnat et al. [ 11], namely the median rank scores (MRS), to derive numerically comparable quantities from the expression values of both platforms used in the different studies.
This computation is equivalent to calculating E [ G| W, Ψ t)], E [ S| W, Ψ t)], and E [ Z| W, Ψ t)] and then inserting these quantities into the expression for ln ℙ(W, G, S, Z|Ψ), such that Equation A1 is a function of parameters Ψ =.
To correct for differences in both RNA quality and quantity between samples, the expression levels of transcripts were normalised to the reference GAPDH gene transcripts.
With proper equalization quantity, the visual impression closely corresponds to the normalized quantities shown in the expression profile chart (Fig. 16c).
In mRNA analysis, QBasePLUS version 2.4 software (Biogazelle BE, Belgium) [ 13] was used to calculate efficiency-corrected relative quantities normalized to the expression of reference genes for all samples (NRQ).
For example, 1) High-temperature stress can not only cause the change in the expression quantity of antioxidation-related genes, but also lead to the change in the expression quantity of carbon metabolism-related radical/protein, finally increasing the rice chalkiness [ 5, 45] (Lin et al 2005; Yamakawa et al 2007).
High-density fermentation was applied to the protein to further enhance the expression quantity.
Compared with the shake-flash culture, the expression quantity via high-density fermentation increased by approximately 12-fold.
