Sentence examples for quantity spot from inspiring English sources

Although this protocol can control for differences in target DNA quantity, spot morphology, and uneven hybridization, it cannot normalize labeling and sequence-specific hybridization differences among transcripts [ 8].

Perhaps the most important innovation is in the software, which can now create 3D images, count objects (cattle, plants, warehouse stock (RFID)), recognize plant diseases, estimate ore quantities, spot shark and deploy flotation devices in bathing areas, etc.

For comparison of spot quantities, spot volumes were normalized as a relative volume (% vol, a percentage of the total volumes of all of the spots in the gel).

An intuitively appealing way to do this is to create 2D gels with serially diluted quantities of commercially available proteins, establishing the relationship of protein quantity to spot intensity and then proceeding to compare different statistical pre-processing techniques to the datasets acquired from these gels.

Use a minute quantity of spot fabric remover solution to ease the process.

Cy5 labeled 18S rRNA probe was also included in the hybridization for normalization of differences in RNA quantities per spot.

Since the synthetic RNAs were diluted in total RNA, Cy5 labeled 18S rRNA was also used for normalization of differences in RNA quantities per spot (not shown).

The mean of log ratios method of normalization calculates the normalization factor of a gel by calculating the mean of all log ratios (log spot quantity of gel/log spot quantity of master gel) of all matched spots (master gel – gel).

Spot volume values were normalized in each gel by dividing the raw quantity of each spot by the total volume of all valid spots included in the same gel.

The quantity of each spot was normalized by total valid spot intensity.

The individual protein spot quantity was normalized as follows: the raw quantity of each spot in a member gel was divided by the total quantity of the valid spots in the gel, and normalized spot intensities were expressed in ppm.