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DNA was fragmented by sonication (Branson Sonifier B-12: output control to microTip limit, 35 times at 5 s intervals); verification of the successful fragmentation in term of size and quantity was verified on 1 % agarose gels.

After one round of amplification of 300 800 ng of total RNA, RNA quality and quantity was verified (Agilent Technologies, Waldbronn, Germany).

Quality and quantity of RNA was verified by measuring the absorbance and the A260/A280 ratios were always above 1.8.

The quantity of RNAs was verified by an ultraviolet spectrometer (OD260 /OD280 ratios with 1.84 to 2.04).

The integrity and quantity of RNA were verified by ethidium bromide staining of the 28S and 18S ribosomal RNA bands.

Quality and quantity of RNA was verified by spectrophotometry and the A260/A280 ratios were greater than 1.8.

The quantity of RNA was verified by an ultraviolet spectrometer and electrophoresis on a denaturing formaldehyde agarose gel.

The loading quantity of protein was verified by staining the same membranes with monoclonal anti- α-tubulin or antivinculin antibody (Sigma-Aldrich, Dorset, UK; anti- α-tubulin: 1 : 2000; antivinculin: 1 : 1000) and HRP-conjugated sheep anti-mouse secondary antibody (1 : 5000; Amersham Pharmacia Biotech, NJ, USA).

The quality of completed libraries (insert size and quantity) was verified using an Agilent 2100 Bioanalyzer.

RNA quantity was initially verified using a Qubit fluorometer (Life Technologies).

The quantity of protein loaded was verified by staining the same membranes with GAPDH antibody (1 : 2000, Sigma-Aldrich, Dorset, UK).

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