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Azimuthal tailing of reflections monotonously increased with the increase of the quantity of transformation cycles (Figure 1A,B,C,D).
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Proper methylation of pDH10 prior to transformation is expected to significantly increase the quantity of transformants.
This is the case of the process developed in the present work that will enable the routine transformation of embryogenic cultures derived from leaves selected on the mother tree and the subsequent regeneration of large quantities of transformants from numerous independent transformation events.
The GFPM is designed mainly as a policy analysis tool to project the general future trends in quantities and prices at different stages of transformation under different scenarios.
This means that the quantity of catalyst required for the transformation of an inert electrode into an active one decreases strongly with decreasing active particle size.
Because our initial efforts at transforming Ap have required a substantial amount of in vitro culture (e.g. to generate sufficient quantities of bacteria for transformation experiments and to cultivate potential transformants), we have been concerned that the transformants that arise will be poorly infective for animals.
All plasmids were constructed in Saccharomyces cerevisiae FY2 [ 49] via yeast mediated homologous recombination [ 50], isolated from yeast with a Zymoprep Yeast Plasmid Miniprep II (Zymo Research, Irvine, CA) and transformed into chemically competent Invitrogen E. coli TOP10 (Invitrogen Corp ,Carlsbad, CA) to generate sufficient quantities of plasmid for transformation into T. saccharolyticum.
Rickettsial load in fleas and the ratio of Rf17kDa/Cf18S were assessed after the logarithmic transformation of the quantity of the genes of interest (Rf17kDa and Cf18S).
To determine R. felis-infection density Rf17kDa/Cf18S ratios for individual flea samples were calculated after the logarithmic transformation of the quantity of the genes of interest (Rf17kDa and Cf18S).
The ache of transformation.
A kind of transformation".
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