Sentence examples for quantity of the template from inspiring English sources
In brief, we determined the quantity of the template at the Ct (target cycle) and the efficiency (X) by a internal standard control and the fold increase was calculated as X inputCt-IPCt).
The higher the starting quantity of the template, the earlier a significant increase in fluorescence is observed (Bieche et al, 1999).
The higher the starting quantity of the template, the earlier a significant increase in fluorescence is observed (Wittwer et al, 1997; Bieche et al, 1999).
The ratio of the CHEK2 copy number to CFTR copy number in each sample normalised the results with respect to differing starting quantity and quality of the template DNA in each reaction [ 46].
Threshold cycle (Ct) data obtained from running real-time RT-PCR was related to a standard curve to obtain the starting quantity (SQ) of the template cDNA, and the values were normalized against 18S rRNA [GenBank:AF102857].
The higher the starting quantity of template, the earlier the attainment of the threshold cycle, which is defined as the fractional cycle number at which fluorescence passes a fixed threshold above baseline (Bieche et al, 1998, 1999).
The time (Tt) required for the turbidity of the LAMP reaction solution to exceed a given value was dependent on the quantity of the initial template DNA.
To enrich the quantity of the cDNA template, fifteen cycles of PCR amplification were performed using PCR primers PE1.0 and PE2.0 with Phusion DNA Polymerase.
Curves were generated by plotting the log 10 of the quantity of each template dilution against the C T value obtained during amplification of each dilution.
Both TAIL-PCR and FPNI-PCR methods demand only extremely modest levels of quantity and purity of the template DNA, thus, they can work well with both cell lysates and crude DNA extracts.
The PCR cycle associated with exponential growth of product is tightly associated with the quantity of the initial cDNA template, providing an estimate for the level of mRNA expression in the tissue.
