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Fig. 3 The determination of the minimum quantity of the substrate peptide.
However, the utilized quantity of the substrate formulation containing 25%% did not differ from that of the RRS (positive control).
Five minutes later, the colorization on test zone was used to determine the minimum quantity of the substrate peptide used in digestion step.
The utilized quantity of the substrate formulation containing 25%% was significantly less than RCS (positive control) only in the second trial.
In this work, the minimum quantity of the substrate peptide was found to be 0.2 ng/μL, but the intensity of the color was visibly less clear than that of 1 ng/μL.
However, the excess quantity of the substrate peptide would inevitably result in prolongation of the enzymolysis time thus reducing the sensitivity of the assay within the given digestion time.
Similar(54)
Decomposition rates in the rotations with arable crops were not higher than those for permanent grassland indicating that tillage did not affect decomposition rate and that SOM dynamics were dominated by the quantity and quality of the substrate input.
Complete transglycosylation of the IgG-Fc with Endo-A required a large excess quantity of the donor substrate.
To generate the required ATP, a proportional quantity of the reaction substrate must be catabolized.
Another explanation could be the quantity of the collagen substrate used in different experiments.
The pattern of difference established in structural studies of language in the typical population is that a higher quantity of the neural substrate being measured represents better performance on a given task.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com