Sentence examples for quantity of template from inspiring English sources

Using this technique, the amount of organic template is consumed in the synthesis (occluded in as-synthesized MCM-41), thus can be estimated (Note: the quantity of template lost during the purification process of the samples is also taken into account).

Two normalization steps were used: the first normalization consisted in loading the same quantity of template RNA in each well and the second in normalizing the data against an endogenous gene.

Additionally, there is a higher likelihood of nonspecific DNAs preferentially amplified due to the lower quantity of template viral DNA as opposed to single bacterial cells as a result of the significant difference in particle (cell) size and genomic DNA content (25 100 nm; ∼1.5femtograms for viruses, as opposed to 0.2 1.5 um; ∼14femtograms for bacteria).

These reaction conditions were empirically determined by optimizing the annealing temperature, MgCl2 concentration, quantity of template and number of cycles.

Serial dilutions of a known quantity of template for each gene were used to measure the copy numbers.

It is also the most suitable for expression analysis of miscellaneous cytokines from a single sample and a small quantity of template material.

In real-time quantification of PCR assay, the Ct value is a parameter reflecting the quantity of templates presented in the reaction [ 25].

To correct for differences in the quality and quantity of templates, ligation frequencies between the fragments were normalized to a fragment in the human Ercc3 locus.

In some cases, two rounds of PCR were needed to produce sufficient quantities of template for sequencing.

Despite the wide recognition of the importance of phage, the field of marine phage genomics, as well as phage biology in general, have suffered from technical limitations including (i) limited prior knowledge of the target genome sequence and (ii) restrictive quantities of template DNAs.

WGA can be used to generate sufficient DNA for genotyping using large quantities of template.