Sentence examples for quantity of starting from inspiring English sources

Our results illustrate that it was not critical to use a large quantity of starting material to achieve an adequate amount of purified protein either to improve an immunoassay or to characterize a protein chemically.

Threshold cycle numbers for the various PCRs were compared to the appropriate standard curve to calculate a relative quantity of starting cDNA for each housekeeping gene and each target gene within each individual sample tested.

Kiss1 expression was measured based on the amount of total RNA extracted from the tissue which is known to have a linear correlation (r2 = 0.99) between the quantity of starting tissue sample and the yield of purified RNA extracted by a Trizol method (Invitrogen, Carlsbad, CA).

This often results in amplification failure when the quantity of starting material is limited.

Since expression levels decrease as the quantity of starting RNA decreases, it is not possible to assess the change in variance as the quantity of starting RNA decreases, without simultaneously considering the level of expression.

This method works successfully from a limited quantity of starting material and eliminates the requirement for genome-specific PCR amplification.

When conducting RNA-Seq studies, one potential obstacle encountered by researchers can result from suboptimal starting quantities of RNA that the use of NGS platforms often requires.

The ratio between the relative amount of NOS1 and β-actin was then calculated to compensate for variations in quantity or quality of starting mRNA as well as for differences in reverse transcriptase efficiency.

With attention to primer design and cycling conditions, virtually any mRNA species can be accurately quantified from even minute quantities of starting RNA.

Because large amounts of biological material are often challenging to collect, tremendous efforts have been made for a decade to design miniaturized platforms such as lab-on-a-chip or microarrays to run sensitive and reliable analysis from tiny quantities of starting material.

Low quantities of starting RNA can be a severe hindrance for studies that aim to utilize RNA-Seq.