Spike density threshold is estimated as the 95% percentile for a random Poisson process producing the same quantity of spikes.
To assess the accuracy of the MIRALCS, we added a known quantity of ERCC spike-in mRNAs.
An appropriate quantity of Mo Mo double spike was added (based on screened concentrations) and weighed by evaporation correction.
For a pair of cells with overlapping firing fields, we identified each cell's spikes within its firing field for each individual lap, and computed two quantities of these spikes: the (within-field) firing rate and the center of mass (COM) of their firing locations.
The quantity of the yeast spike-in mix was optimized to avoid interference with the correct amplification of the human samples.
For the purpose of a positive control, a 45.5 kg quantity of feed was also spiked with 100 mL of stock PEDV (Ct = 25.22) along with 147.42 mL of sterile saline.
The period leading up to the English Civil War saw a huge spike in the quantity of pamphlets published.
The aqueous standard was prepared by spiking a measured quantity of methanol standard into a 100 mL volumetric flask filled with reagent water.
For DNA fragments generated by ChIP (or blunt-end restriction) these limitations are effectively overcome by prior phosphatase treatment, by using recombinant terminal transferase enzyme, and by spiking a small quantity of terminating nucleotide into the tailing reaction.
An aliquot of control pool D was reconstructed to form artificial 'case' pools by replacing 15 and 20% of the pool sample with the same quantity of DNA of one genotyped individual (the spike).
