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Moreover, the large quantity of reads allowed a draft genome for 15 species to be assembled.
These libraries are non-normalized, so we estimated the relative gene expression based on the quantity of reads.
When calling variants (especially from short reads), the quantity of reads and the accuracy of mapping the reads should be precise.
Efficient base calling by using the latest Illumina data analysis pipeline software improved the quality and quantity of reads from the same raw image data.
Although 3-prime ends of transcripts were found to be represented by a somewhat higher quantity of reads, the overall coverage shows a largely even distribution and sufficient coverage of the coding transcript sequences by RNA-Seq reads.
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We value quantity of reading over quality of reading.
The quantity and quality of reads for each library are shown in Additional file 1: Table S1.
Then, HLA-VBSeq optimizes both read alignments to the HLA allele sequences and relative quantities of reads on HLA alleles simultaneously under a statistical framework by variational Bayesian inference.
We argue that full V(D J segmentation on these quantities of reads is unnecessary, and that a better strategy for clonality studies is to first cluster the reads derived from the same clone before the time-consuming V(D J segmentation.
This technological evolution was paralleled by the development of new algorithms to deal with the quantity and the quality of reads produced.
A series of Perl scripts was developed and applied to determine the type (substitute, insertion, deletion, synonymous or non-synonymous) and quantity (number of reads per variant) of variation between the four samples and the reference cell line and in between donor and recipient.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com