Ovarian ageing, dictated by a decline in the quantity and quality of oocytes within the ovaries, 3 4 is responsible for the well-established observation of age-related decline in fertility 5 8 and of age-related increase in adverse reproductive events such as miscarriages 9 10 and aneuploid pregnancies.
This study aims to evaluate the hypothesis that DHEA supplementation for at least 12 weeks prior to and during controlled ovarian hyperstimulation in patients predicted to be poor responders increases the oocyte quantity (number of oocytes retrieved).
Each analyte was first evaluated with covariate factors known to influence outcome, such as age, attempt rank, number of oocytes collected, quantity of gonadotrophins administered during the stimulation and the estradiol level on the day of hCG injection.
In consonant with the notion that MagT1 protein mediates the observed Mg2+ currents is the association of the magnitude of the Mg2+-evoked current with the quantity of MagT1 protein in oocytes injected with MagT1 cRNA (Fig. 6).
In species like C. gigas, having an "r" demographical strategy, characterized by high fecundity, reproductive success is greatly dependent on the quantity of gametes produced, especially oocytes, as well as their quality.
The principal objective of this study is to evaluate the effect of DHEA on the ovarian response to gonadotropins (oocyte quantity) during controlled ovarian stimulation and the developmental competence of oocytes (oocyte quality) by using molecular and clinical markers in women with aged ovaries, identified by using reported AFC and AMH thresholds.
In line with the vast majority of published studies evaluating the value of AMH as a predictive marker in ART treatment (reviewed by Broer et al., 2010; La Marca et al., 2010), this study demonstrated that serum AMH is a predictive factor of oocyte quantity rather than quality, irrespective of the gonadotrophin preparation used.
The number of oocytes retrieved and basal FSH were similar between groups, so differences in oocyte quantity or quality cannot explain the improvement in per-transfer OPR with CCS-SET.
As a control, the same quantity of inhibitor (184 nl corresponding to 20 oocytes worth of inhibitor) was added to 5 ml of buffer (36.8 nM final) prior to addition of 20 oocytes and 5 min incubation.
The results in Figure 4 show that the rate of L-lactate uptake was approx. 50% inhibited in the AR-C155858-injected oocytes, whereas the same quantity of inhibitor added outside was without effect.
