Sentence examples for quantity of mutant from inspiring English sources

This data supports our hypothesis that synthetic peptides designed to represent the variable regions of HIV-1 envelope should induce immunity against a large quantity of mutant strains.

In PCR assays designed to detect rare somatic mutations, SuperSelective primers, by virtue of their short 3′-foot sequences, selectively initiate synthesis on mutant DNA target fragments, while suppressing the synthesis of related wild-type fragments, and the resulting threshold cycle reflects the quantity of mutant targets present.

Yet such proof-reading polymerases could introduce biases when the quantity of mutant molecules in the template DNA is low.

In HET mice, the relative quantity of mutant desmin was within a range of 20 50 % in both skeletal and cardiac muscle tissue.

However, in the subsequent analyses, the time of data collection was ignored as a factor that potentially influenced the quantity of mutants.

Normal quantities of mutant HN in the cytoplasm and on the surface of infected cells were detectable (Fig.  1a, c).

Following PCR amplification, absolute quantities of mutant and WT DNA copies were determined using the QuantaSoft software as previously described.

Recently, knock-in animal models expressing physiological quantities of mutant APP have been generated to help differentiate between synaptic and cognitive effects caused by overexpression and those by mutation of APP.

When equal quantities of mutant and WT DNA were used, we observed the same number of peaks with both nucleases: two peaks were distinguishable for exon 2a and three for exons 3 and exons 6.

To determine the sensitivity of the COLD-PCR melting curve assay to detect low quantities of mutant allele in WT background we adopted two complementary strategies: (1) we mixed KRAS-mutant cell line DNA with KRAS-WT peripheral blood leukocyte DNA, and (2) KRAS-mutant FFPE tumor DNA with KRAS-WT FFPE tumor DNA at mutant:WT ratios of 50/50, 20/80, 10/90, 5/95, 2/98, and 1/99.

This technique may be useful for obtaining milligram quantities of mutants of a protein of interest or for evaluation of several mAb clones.