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Purified RNA samples were then analyzed by Nanodrop 1000 and Agilent 2100 Bioanalyzer RNA Nano Kit for the determination of the quantity and integrity of RNA.
In order to avoid experimental errors arising from variation in the quantity and integrity of the RNA template, as well as the efficiency of the cDNA synthesis and PCR amplification, a normalization step is an essential pre-requisite.
The quantity and integrity of the DNA extracts was monitored through spectrophotometer readings and amplification of β-globin gene (endogenous control).
Items such as quality, quantity and integrity of the cartilage graft were included in a histomorphological grading system to provide information about the properties of a specific cartilage graft.
The quantity and integrity of poly(A)+ RNA was determined by the same measures used for 5' capped RNA.
The quantity and integrity of isolated RNA was determined using the Agilent 2100 Bioanalyzer (Agilent, Palo Alto, CA, USA).
The quantity and integrity of the RNA was measured with NanoDrop (ThermoScientific) and used 3 µg RNA as template for SuperScript III RT (Invitrogen) and oligo dT 12 18 (Invitrogen) as specific primer for amplification of mRNA.
The quantity and integrity of the samples were determined with NanoDrop 2.5.4.
Quantity and integrity of RNA were assessed by spectrophotometry and 0.8% agarose gel electrophoresis, respectively.
Quantity and integrity of the RNA were verified by using RNA 6000 nano chips (2100 Bioanalyzer; Agilent, Palo Alto, CA, USA).
This method used amplicons of 3 different sizes (41 bp, 129 bp and 305 bp) to estimate the quantity and integrity of amplifiable DNA.
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