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The variation in the quantity of identified peaks indicates that default stringency levels are tuned differently among programs.
However, the comprehension of miRNA function remains preliminary, because few miRNA targets have been identified in spite of the enormous quantity of identified miRNAs[5], [29], [30].
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Peptide counts were assigned to each protein identified through mass spectrometry by measuring the quantity of the identified peptides by their spectra, and filtered by those peptides that also occurred in negative control experimental samples.
We estimated the quantity of each identified protein from the respective spot area on the 2D gel.
The quantity of MITEs identified in the remaining genome types was less than 6% of reads except for the notable exception of the distantly related species O. brachyantha [FF; 362 Mbp] with 15.28% of reads.
However due to a combination of (1) the quantity of variants identified and (2) the requirement for large numbers of genotyped individuals to attain sufficient power in the association analysis renders this a costly approach.
These were defined according to the following expressions, where Vp is the actual number quantity of objects identified by the physician, f n being the quantity of objects, which were not marked by the strategy and f p being the number of spurious regions, which were marked as objects.
These are defined according to the following expressions: where V p is the actual number quantity of lesions identified by the physician, f n being the quantity of lesions, which were not marked by the algorithm, and f p being the number of spurious regions, which were marked as lesions.
This large difference of detected relative WT quantity suggests that most of identified peptides for WT p94 by the iTRAQT™ method are derived from extensively autolyzed fragments of p94 that could not be detected by Western blot analysis due to small molecular mass and heterogeneity of the autolyzed fragments (Fig. 5D).
The quantities of the identified compounds are expressed in mg/100 g DW.
The iBAQ [ 43] method has been used to roughly estimate the absolute quantities of the identified proteins.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com