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However, current methodologies are limited in terms of the capacity to isolate and expand a sufficient quantity of endogenous antigen-specific Tregs for therapeutic intervention.
To minimize the quantities of endogenous cytokines, growth factors, matrix metalloproteinases (MMPs) and tissue inhibitor of metalloproteinases (TIMPs) present in the medium, cultures were maintained in the presence of a low serum concentration (1%) the 3 last days before the analysis of secretion profiles of Fp and Fr (from day 6 to day 9).
Humans produce and excrete large quantities of endogenous estrogenic hormones.
We have shown that despite the high quantities of endogenous Rh1, SERT is expressed in similarly high amounts.
Scaling of input material to immunopurify similar quantities of endogenous and overexpressed PPP1R15A led to recovery of similar amounts of associated endogenous actin.
Nociceptor excitability depends on physical stimuli, physiochemical milieu, and the quantities of endogenous pain substances being secreted [ 2– 6].
Relative expression levels of target genes were normalized to the relative quantities of endogenous control genes using geometric averaging with the geNorm VBA applet [ 35].
Poultry species do not produce meaningful quantities of endogenous phytase, the enzyme that can hydrolyse the ester bonds between the phosphate groups and the inositol ring in phytates.
The occurrence of those dysfunctions depends on endogenous factors (endogenous hypothermia) or accidental factors (unintentional hypothermia) [ 4, 5].
This endogenous factor is our main variable of interest.
Relative quantity (RQ) was obtained by dividing the target quantity with the quantity of the endogenous control in each sample.
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