Exact(5)
For bootstrap ANOVA, the pseudogroups were generated from the relative quantity of each transcript or miRNA tested.
The relative starting quantity of each transcript was determined using the comparative CT method for relative quantification [ 36].
Calculation was performed by second derivative maximum analysis and the quantity of each transcript was expressed as ct values.
The quantity of each transcript was normalized to the quantity of the housekeeping genes Glyceraldehyde 3 phosphate dehydrogenase 1 (Gandh1 ) Actinctin 5c (Act5c).
The quantity of each transcript was normalized to the quantity of the housekeeping genes Glyceraldehyde 3 phosphate dehydrogenase (GAPDH) and Actin Beta (ACTB).
Similar(3)
Baseline quantities of each transcript were calculated as the average of experiment 1 animals collected before cholesterol feeding (n = 18) and experiment 2 animals after 0.002 mg/kcal cholesterol feeding (n = 5).
Relative quantities of each transcript were normalized by a housekeeping gene (GAPDH) and expressed as fold increase versus control.
We performed semiquantitative real-time PCR (RT-PCR) to assess relative quantities of each transcript with the 7500 Real-Time PCR System (Applied Biosystems, Foster City, CA).
Related(1)
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