Sentence examples for quantity of each gene from inspiring English sources

First, we calculated the relative quantity of each gene g and sample i with the delta-Ct formula [37].

Unlike FACS, the algorithm used here cannot differentiate between a population of a given cell type that are all homogeneously mildly activated and a population of that cell type that consists of a mixture of some resting cells and some strongly activated cells, since the parameters used to assess activation are merely the total quantity of each gene's mRNA present in the sample.

The geometric mean was calculated and the normalized quantity of each gene transcript and standard deviation of the three replicates was then calculated [ 30].

The mean quantity of each gene was divided by the mean quantity of ACTB and was used for the normalization of input DNA.

The mRNA quantity of each gene was calculated with the 2-△△Ct method and normalized to the abundance of a house-keeping gene, Bmβ-actin (GenBank NO: EU780706.1).

The relative quantity of each gene in the asbestos-treated group compared with the corresponding gene in the control group was calculated for all samples using 18S rRNA (ribosomal RNA) and GAPDH (glyceraldehyde 3-phosphate dehydrogenase) as endogenous controls.

Therefore, we hold that the gene quantification was reliable in this experimental condition when the gene expression was at medium and high expression levels.> To identify significant DEGs during petal development, the expression quantity of each gene in three petal libraries was compared pairwise and filtered with |log2 fold change)| > 1 and q value < 0.005.

The relative quantity of each gene's transcript was calculated using the ΔCt method (Bio-Rad Application Guide) to normalize to the quantity of the GAPDH transcript.