Curves were generated by plotting the log 10 of the quantity of each template dilution against the C T value obtained during amplification of each dilution.
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To assess RNA quality and quantity, several dilutions of each sample were analysed using the Agilent RNA 6000 nano Kit and Agilent Bioanalyzer 2100.
Using the Sterne strain as a surrogate of anthrax infection, the quantity of bacterial cells present in a dilution series was established.
Variations in biomarker detection in favour of ECoScreen2 could not simply be attributed to variations in the dilution as the quantity of condensate was also significantly increased.
Different cDNA samples were amplified with primers for 16S rRNA, and the cDNA samples were normalized by differential dilutions according to quantity of 16S rRNA products.
Transcript levels were calculated using the standard curve method (User Bulletin #2, ABI Prism 7700 Sequence Detection System, Applied Biosystems) made from serial dilutions of known quantity of plasmid (10, 10, 10, 10, 102 copies/μl).
Five different quantities of the target sequence (10,000, 100,000, 150,000, 200,000, and 1,000,000 molecules) were amplified in the presence of dilution series of the competitor sequence.
A quantity of 100 μl of serum-free medium, TLR2 agonist dilution or water dilution was added to a well of a 96-well plate, with each condition represented in duplicate wells.
The satellite-receiver geometry can be characterized by a unit-less quantity called Geometric Dilution of Precision (GDoP).
