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The minimum quantity of buffer needed to obtain a constant pH at the reaction site was also determined.
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In the network environment, the average system queue size corresponds to the Average Quantity of Buffered Traffic (AQBT), the job's arrival rate corresponds to the total traffic generation rate, and the average waiting time corresponds to the average delay, which means that the following holds.
Because less water in an industrial process minimizes waste, we tested the SGase reaction with varying quantities of buffer in crude biodiesel (Fig. 4f).
The pH of Tris-HCl in this modified MNase digestion buffer is high because oocyte lysates are acidic and we used a limited quantity of the buffer so as not to dilute histones in samples (50 whole injected oocytes in 100 μl buffer).
Our results also show that nature and quantity of dilution buffer are important for increasing efficiency of direct PCR.
Around 40 50 µg of caseous content were placed in identical cryogenic vials (containing the same amount of beads), followed by the addition of an identical quantity of lysis buffer.
Non-template control reactions were setup by transfer of approximately the same quantity of washing buffer to tubes containing only the lysis solution.
Buffering capacity may be defined as the number of moles of a strong acid or base required to change the pH of a given quantity of a buffer by 1 pH unit [125, p. 123].
About 2.0 g of wheat bran was taken in an Erlenmeyer flask and mixed with a predetermined quantity of Tris buffer (0.1 M, pH 8.0), and a calculated amount of beef extract and NaH2PO4 was also added to the flask.
In contrast, intradermal or intramuscular needle injection induces less cell damage, and tissue nucleases are diluted by relatively large quantities of injection buffer so that the injected DNA can be taken up by cells before its extracellular degradation.
Each sample contained ∼1.5 mg of enzyme (∼81 μL of a 18.5 mg/mL solution) and a sufficient quantity of 20 mM Na2HPO4 buffer (pH 9.0) to give a final volume of 990 μL.
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