However, the fact that PrPres generated in vitro was associated with approximately 10 times less infectivity than an equivalent quantity of brain-derived PrPSc casts doubt on the "protein-only" hypothesis of prion propagation and backs theories that suggest there are additional molecular species of infectious PrP or other agent-associated factors.
After loading similar quantities of brain equivalent tissues on each lane, cleaved PrP, corresponding to the C2 fragment, was only abundant in mice with "C-BSE like" features.
However, the need of large quantities of brain tissue and elaborate protocols to isolate the fibrils have limited its use.
After confirmatory Western blot, insufficient quantities of brain tissue were available for Western blot discriminatory testing in 26 samples, which are shown in the Table as samples not analyzed.
Every top show is like a comedy Los Alamos project - burning up vast quantities of brains, cunning, endurance and paranoia.
The simultaneous communication between billions of neurons produces a large quantity of electric brain activity, which can be detected and measured through EEG technology.
All spectra were processed offline using the LCModel software package [80] to determine the quantities of the brain metabolites N-acetylaspartate and N-acetylaspartylglutamate (collectively referred to as NAA), choline-containing compounds (referred to as Cho), myo-inositol (MI), and creatine-containing compounds (referred to as Cr).
In reducing the quantity of oxygen the brain receives during sleep, sleep apnea can cut down on the amount of nourishing "deep sleep" a person gets each night, something that can impair memory and increase the chances of depression.
To evaluate these parameters, equal quantity of protein from brain homogenates of PrPKO and Wt mice was fractionated by SDS-PAGE and immunoblotted (Figure 1A).
To assess the expression levels of PrP in PrP+ mice relative to Wt controls, an equal quantity of protein from brain homogenates of Wt, PrPKO, and PrP+ mice was fractionated by SDS-PAGE and immunoblotted.
