Sentence examples for quantity of beads from inspiring English sources
When THF/DMF ratio was less than 1 2 (v/v), beaded nanofibers with a rough surface were produced, while the quantity of beads was less than that of nanofibers from larger THF/DMF ratios.
To standardize fluorescence, a known quantity of beads calibrated in fluorescein equivalents (Bangs Laboratories, Fishers, IN) were filtered in lieu of bacteria.
For quantification, samples included a known quantity of beads (TruCOUNT™, BD Biosciences, San Jose, CA, USA) of 4.2 μm diameter.
A known volume of crude extract was used to perform binding with an optimal quantity of beads for 30 minutes to 2 hours depending on the kit used, at 4°C with gentle rotation.
The amplified beads were first run on a workflow analysis slide to determine the quality and quantity of the beads, which was followed by a single sequencing run, performed according to the Applied Biosystems SOLiD 4 System Instrument Operation Guide.
After incubation, 800 μl of H/H buffer pH 7.4 plus 100 μl containing a defined quantity of TruCOUNT™ beads (BD Biosciences, San Jose, CA) was added to each sample tube prior to analysis by flow cytometer.
Around 40 50 µg of caseous content were placed in identical cryogenic vials (containing the same amount of beads), followed by the addition of an identical quantity of lysis buffer.
Following emPCR, approximately equal quantities of enriched beads from each library were pooled into a single library, and sequenced on a full PicoTiter plate using LR70 chemistry.
Compared with terrestrial conditions, lunar gravity reduced the total quantity of 0.5-μm beads settling in the airways by 25% and 1-μm beads by 32%, explains first author Chantal Darquenne, an associate professor in the Department of Medicine at the University of California, San Diego.
Cells were stained for DX5 and CD3 prior to quantifying by flow cytometry by comparison to a known quantity of CountBright Absolute counting beads (Molecular Probes, Invitrogen, USA) on a Cyan ADP LX 9 color flow cytometer (DAKO, UK).
