The quantity of immunoreactive bands at 94 kDa (full-length protein) was determined by densitometry and expressed as a percentage of control.
The quantity of each band is expressed as total pixel value, based on intensity and number of pixels per band.
The quantity of a band was measured by the area under its intensity profile curve using BioRad Quantity One 1-D Analysis Software Bio-Rad Bio-Rad Laboratoriesles, CA, USA).
The trace quantity of a band is represented as c.p.m.
The trace quantity of a band was measured by the area under its intensity profile curve (units=intensity × mm).
Image quantitation was performed with Quantity One version 4.6.7 using default options (all lanes, auto detect = yes, normalize = yes, sensitivity = 10, size scale = 5) and reporting relative quantity of each band per lane.
To normalize the signal intensities, the total raw quantity of each band was divided by the average intensity value of the molecular standards in that image and the normalized values for the duplicate samples were averaged and expressed as normalized intensity units (i.u).u
His tools are bookbinder's glue, a metal instrument called a micro-spatula, a tongue-depressor-like implement called a bone folder, an X-Acto knife, rice paper, book cloth, acid-free tissue, and quantities of rubber bands, which he says are key to the operation.
The relative quantity of the protein bands was determined by an image analysis system (Odyssey 3.0 software).
The quantity of RT-PCR bands were analyzed based on the intensity under Gel Doc 2000 software (BIO-RAD).
The quantity of each reamplified bands were checked on a 1.8% agarose gel against the 1650-bp fragment of the DNA ladder 1 Kb plus (Invitrogen).
