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Referred to as "optical calibration", this can be accomplished by amplifying a known quantity of a standard and dividing the resulting F0 value by the predicted target quantity, expressed as nanograms of amplicon DNA (M0).
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Another approach for TAG measurement is also used and consists in the addition of different quantities of a standard to an algal culture to determine its lipid concentration [ 105].
The aqueous standard was prepared by spiking a measured quantity of methanol standard into a 100 mL volumetric flask filled with reagent water.
Since [H3O+] must also be known, the commonest procedure is to measure [A]/[B] in a solution made by adding a small quantity of A or B to a standard buffer solution.
In this paper we highlight that when agents can choose both the quality and the quantity of a given good/service, this standard commodity tax result needs to be qualified.
Afterwards, an adequate quantity of iodine standard solution is added, and then sealed and preserved under a dark place for at least 5 min.
Of the remaining 44 teeth, 22 then were instrumented with GT and Profile (Dentsply/Tulsa Dental Co, Tulsa, Okla) instruments to apical size #35 (group 1) and 22 teeth with Pow-R instruments (Moyco/Union Broach, York, Pa) to apical size #50 (group 2), in the presence of a standard quantity of phosphate-buffered saline solution placed in the canal.
To determine RT PCR efficiency and initial starting quantity of the samples, a standard curve was generated using samples from a 1 : 3 dilution series of total starting cDNA.
The International System of Units (SI) and its associated methods adhere to a design principle in which all kinds of quantities are described in terms of a standard set of dimensions.
They amplified the tiny sample into a large quantity of DNA using a standard technique called whole genome amplification.
The spot intensities were converted into the quantity of ATP using a standard curve generated with [α-P]ATP.
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