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We further checked the effect of two experimental parameters on subjects' RT: (1) number of objects within the test set which represents the amount of relevancy load (figure 3a) and, (2) amount of difference between objects quantity in the sample and test sets which represents the amount of irrelevancy load (figure 3b).
Because of the quantification and the normalization of the spot intensity, one should realize that the relationship between the original protein quantity in the sample and the measured spot intensity is affected by various intervening factors.
In addition, fragment peak height is a direct function of the absolute quantity in the sample, such that the relative heights of the corresponding "methylated" and "unmethylated" peaks provide a quantitative score of methylation of the corresponding "CpG unit".
Moreover, correlation depends on the range of the true quantity in the sample, with wide ranges giving greater correlations than narrow ranges, which has nothing to do with whether the true agreement is high or low.
However, the relation between original protein quantity in the sample and measured spot intensity is influenced by various intervening factors: loss of sample during entry into the IEF gel, efficiency of transfer from first to second dimension, protein loss during staining, staining efficiency, a protein's staining curve over time, staining curve over concentration, and dye bleaching.
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After neutralization the reduced sugar quantity in the samples were measured by the method of Miller (1959) with little modifications. 1 ml DNS solution was mixed with 1 ml of the neutralized algae juice in Eppendorf tubes and the mixture was heated at 100 °C on hot block for 10 min.
For those that were detected in culture, the median quantity in the samples missed by qPCR was 7.9×103 cfu/ml (IQR, 2.0×103 – 7.9×104) which was lower than for those detected by qPCR [4.0×106 cfu/ml (IQR, 1.0×105 2.0×107), p<0.01].
These findings suggest that the cause of negative PCR results was neither the detection limit of yeast DNA for the test nor insufficient DNA quantities in the sample.
The quantity we want to measure in the sample is described by the function f x,y,z), which corresponds to the absorption coefficient in optical absorption tomography and to the refractive index in the case of optical phase tomography.
The CT was used for kinetic analysis and was proportional to the initial number of target quantity copies in the sample.
Using the standard curve of the serial dilutions of ssDNA_ODN, the unknown quantities in the samples were calculated.
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