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The PAs detected were similar to those reported previously (Betz et al, 1994), and included symphytine, 7-acetyllycopsamine, and 7-acetylintermedine as major components in near equal amounts; intermedine and lycopsamine were present in relatively smaller quantity (data not shown).
As with all our constructs, the preparation was analyzed by size exclusion chromatography (SEC) to ensure that we were examining un-aggregated protein of the correct size and quantity (data not shown) and confirmed by dynamic light scattering.
We did not see differences in DNA quantity (data not shown), however more research is required to see if there is a difference with quantity as well as quality.
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In the remaining 34 samples the repeated extraction with the PCI method did not result in higher DNA quantities (data not shown).
Isotype determinations with the experimental samples made with known PC-tubulin were, within error, the same as the expected quantities (data not shown).
Transduction efficiency of TAT-pK was easily influenced by several factors, such as pH or temperature of medium, preincubation period, and quantity of DNA (data not shown).
LIM1863 cells also have the advantage that they, like many human CRC cell lines, do not synthesize detectable quantities of PGE2 (data not shown), thereby allowing us to easily manipulate cell exposure to PGE2.
The essentially same scores were obtained when the combined fusion markers were re-ranked based on the highest quantity in each sample (data not shown).
In this situation, the percentages of positive cells calculated by subtracting positive and negative histograms did not reflect the true fraction of positive cells and changes according to the quantity of antibody added (data not shown).
Then even after we removed 50 genes with the largest Ks value, the relationship between the evolutionary rate and the characteristic quantities remains the same (data not shown).
Our experimental results show that strain SM-A87 can produce large quantities of capsular polysaccharide (data not shown).
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