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Items such as quality, quantity and integrity of the cartilage graft were included in a histomorphological grading system to provide information about the properties of a specific cartilage graft.
The quantity and integrity of poly(A)+ RNA was determined by the same measures used for 5' capped RNA.
The quantity and integrity of isolated RNA was determined using the Agilent 2100 Bioanalyzer (Agilent, Palo Alto, CA, USA).
Purified RNA samples were then analyzed by Nanodrop 1000 and Agilent 2100 Bioanalyzer RNA Nano Kit for the determination of the quantity and integrity of RNA.
The quantity and integrity of the RNA was measured with NanoDrop (ThermoScientific) and used 3 µg RNA as template for SuperScript III RT (Invitrogen) and oligo dT 12 18 (Invitrogen) as specific primer for amplification of mRNA.
The quantity and integrity of the samples were determined with NanoDrop 2.5.4.
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Quantity, quality and integrity of each RNA sample was assessed spectrophotometrically with a Nanodrop ND 1000 and by visualising RNA on ethidium bromide stained agarose gel.
The use of control genes β-actin and HPRT identified 127 cases in which cDNA was considered to be of appropriate quantity and integrity for further analysis.
The quantity, purity and integrity of RNA were evaluated using a NanoDrop ND-1000 spectrophotometer (Nanodrop Technologies, Wilmington, DE) and an Agilent Bioanalyzer (Agilent Technologies, Palo Alto, CA).
Quantity, purity, and integrity of RNA isolates were verified using Agilent Analysis.
gF was predominantly characterized by genes that control the quantity, morphology and integrity of neurons and synapses.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com