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Relative quantities were determined using the equation; relative quantity = 2−ΔΔCt.
Cycling conditions were 50°C for 30 min (reverse transcription) followed by one cycle at 95°C to activate the polymerase, and then by 42 cycles of 94°C for 15 sec and 60°C for one min. Absolute quantities were determined using the standard curve.
Relative quantities were determined using the equation: RQ = 2−ΔΔCT.
Quantities were determined using the relative standard curve method.
Protein quantities were determined using the BCA Protein Assay kit (Pierce) with BSA as the standard.
Relative quantities were determined using the equation relative quantity = 2−ΔΔ C t.
Similar(49)
RNA quality and quantity were determined using a Nanodrop 2000 spectrophotometer (Thermo Fisher Scientific).
The dye-incorporation ratio and the cRNA quantity were determined using the Nanodrop spectrophotometer.
RNA was isolated using the RNeasy mini kit (Qiagen) and quality (260/280 nm ratio) and quantity were determined using a Nanodrop® ND-1000 system (Witec AG, Switzerland).
The A260/280 ratio and quantity were determined using the nanodrop.
The RNA quality and quantity were determined using NanoDrop 2000 (Thermo Scientific) and Bioanalyser 2100 (Agilent).
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