The calculation of the quantities is done by Eqs.
The measurements of the quantities were done on silt loam in a sloping vineyard (Italy) at various times and locations to get a wide range of measured values.
This procedure, combined with a consistent approximation of the geometric quantities, is done to satisfy the discretized mass conservation equation to machine accuracy, as well as to gain the favorable convergence properties of the Poisson solver.
Normalization for cDNA quantity was done using GAPDH control primers for each template.
Total RNA isolation, quality & quantity check was done exactly same as described above for miRNA.
One paired-end (PE) cDNA library was generated from the pooled total RNA of shoot, root, mature leaf and flower buds in equal quantity and sequencing was done in one lane to generate 72 bp PE reads.
Purification of CpCSV-Sy virions and production of a rabbit antiserum to CpCSV-Sy was done using quantities and methods specified elsewhere [ 2].
Interestingly, in one paper (Schmid et al, 2002) where genotyping was done, substantial quantities of a transcript were detected in three cell lines, which were homozygous AA for rs4072037.
Weaving was done in quantity, as evidenced by Spanish accounts; and, more spectacularly, goldsmithing was a major expression of the artist's skill.
Quantitative densitometry of protein bands was done using Quantity One software (Biorad).
