Sentence examples for quantities of viruses from inspiring English sources

For preparation of large quantities of viruses for genome sequencing, two litres of an O. tauri exponentially growing culture (approx. 5.107 cells ml−1) was inoculated with 200 ml of OtV5 lysate (approx. 5×108 pfu ml−1, ratio O. tauri/OtV5 around 1). Lysed cultures were passed sequentially through a 5 μm and 0.45 μm filters to remove large cellular debris.

The quantities of viruses were determined by TCID50 and quantitative real-time RT-PCR targeting the universally conserved matrix gene of influenza A. These viruses were serially 10-fold diluted and the luminescence counts were measured.

For any of these applications is necessary to produce large quantities of viruses, which can be generated in vivo using the natural host insect larvae by per os infection with OBs; or in vitro, using cell lines derived from the same hosts, by infection with BVs.

You have to have serial swabs and try to do semiquantitative PCR or plaquing studies so that you're actually measuring quantities of virus.

In addition, viral plaque assays are widely used to study virus viability, but they are not sensitive enough to detect small quantities of virus.

The quantities of virus RNA were lower, rose more gradually, peaked on DPI 3 4, and declined more rapidly than oral shedding (Table 2).

In both barnyard experiments, introduction of recently-infected mallards was followed rapidly by infection and shedding of virus by contact ducks, and accumulation of substantial quantities of virus in water from the shared pool.

The methods used for detection of KI, WU and MC polyomaviruses, were highly sensitive and should be adequate for the detection of low quantities of virus DNA reaching the detection level of 0.1 virus/copy per cell or less.

The most likely explanation why any of those approaches failed could be less DNA of HBoV, but as long as it is not possible to cultivate HBoV routinely it is difficult to obtain large quantities of virus and one is dependent on high titer clinical specimen.

Trace quantities of virus could be added to tissue homogenates and recovered in a high state of purity.

Swine had become infected by the fecal oral route, with pig feces containing large quantities of virus.