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Both the extracts were exhibited similar quantities of total phenolics.
Therefore, we confirmed that both miRNAs ta-siRNAs can be detected by this method with small quantities of total RNA.
Juices have lower quantities of total phenolics content (TPC), anthocyanins, and antioxidant compared to the whole fruit.
This system permits custom-design options for relative quantification of multiple gene target expression, simultaneously in a single reaction, using nanogram quantities of total RNA template.
This assay can be performed with small quantities of total cellular extracts as a source of enzyme and can be used to rapidly screen virtually any potential inhibitor.
This allows for small quantities of total RNA to be used for the measurement of many individual miRNA molecules.
We optimized the manufacturer's protocol with the following modifications for using small quantities of total RNA from samples.
For the Bonner- and OCT-pools, equal quantities of total RNA from lymph nodes of four or eight individual Mule Deer, respectively, were combined.
Equal quantities of total RNA obtained from neutrophils of five donors were pooled together and purified on QIAGEN RNeasy column (QIAGEN, Mississauga, ON, Canada).
Counts per gene were normalized to average counts of eight polyadenylated RNA spikes that were introduced into fixed quantities of total RNA prior to polyA selection.
Our previous efforts to identify genes regulated by Pax6 in lens used much larger quantities of total RNA (∼5 µg/sample) obtained from 6-week old lenses [80].
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