Pearson's correlation analysis was used to estimate relative degree by quantity of the expression of the three genes.
To screen quantity of the expression of FBPase isozymes in KLN-205 cells, mouse FBP1-specific primers (forward 5′-CAGCTGCTGAATTCGCTCTG, reverse 5′-ACATTGGTTGAGCCAGCGATA) and mouse FBP2-specific primers (forward 5′-GACCCTCTGGATGGATCTTCA, reverse 5′-CAGAAGGCTCATCCTCCGT) were used.
The reporter constructs pRT-LK, pGL3-OT and pGL3-OF were electroporated by nucleofection into U937 cells together with isomolar quantities of the pCDNA3 expression vector encoding the transgenes of interest.
The cells transfected with titrated quantities of the APP expression vector were subject to either thapsigargin treatment, to induce ER stress, or PBA, to stimulate AICD production, or left untreated as the control.
For all candidate genes (Table 1), the quantities of the mRNA expression relative to GAPDH were obtained.
In mRNA analysis, QBasePLUS version 2.4 software (Biogazelle BE, Belgium) [ 13] was used to calculate efficiency-corrected relative quantities normalized to the expression of reference genes for all samples (NRQ).
However, which is responsible for this contribution: the quantity of the Sp1 expression or the binding affinity to p16INK4a promoter?
The relative quantity of the RNA expression was calculated using the comparative CT method with the 7500 System SDS software [ 10].
On the other hand, compared with the measured quantities of gene expression levels in experiments, the numbers of samples are severely limited.
The results indicated that the quantities of XPF expression of BCC and SCC specimens were approximately 57.0 and 76.4% less than that of normal skins, respectively.
All reactions were performed in duplicate, and the quantities of target gene expression were normalized to the corresponding GAPDH expression in test samples and plotted.
