Sentence examples for quantities of template from inspiring English sources
In some cases, two rounds of PCR were needed to produce sufficient quantities of template for sequencing.
Despite the wide recognition of the importance of phage, the field of marine phage genomics, as well as phage biology in general, have suffered from technical limitations including (i) limited prior knowledge of the target genome sequence and (ii) restrictive quantities of template DNAs.
WGA can be used to generate sufficient DNA for genotyping using large quantities of template.
The fold of amplification increases with smaller quantities of template RNA, but the absolute yield of aRNA decreases.
Another advantage of MIRU-VNTR is that it does not require large quantities of template DNA for typing [ 5].
This approach allows the implementation of non-proofreading, highly processive polymerases that limit costs yet generate substantial quantities of template for downstream analyses.
Two samples with known quantities of templates were prepared: one sample contained the pBS sequence template with a cut in the homologous region created by PvuII digestion (cut template) and the intact ssDNA Phagescript DNA (reference template).
Using this technique, the amount of organic template is consumed in the synthesis (occluded in as-synthesized MCM-41), thus can be estimated (Note: the quantity of template lost during the purification process of the samples is also taken into account).
Two normalization steps were used: the first normalization consisted in loading the same quantity of template RNA in each well and the second in normalizing the data against an endogenous gene.
Additionally, there is a higher likelihood of nonspecific DNAs preferentially amplified due to the lower quantity of template viral DNA as opposed to single bacterial cells as a result of the significant difference in particle (cell) size and genomic DNA content (25 100 nm; ∼1.5femtograms for viruses, as opposed to 0.2 1.5 um; ∼14femtograms for bacteria).
These reaction conditions were empirically determined by optimizing the annealing temperature, MgCl2 concentration, quantity of template and number of cycles.
