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Our experiments directly demonstrated that different centrifugation methods produced distinct quantities of plasma microRNAs.
Conversely, dying early might prevent subjects from having a chance to receive large quantities of plasma, which typically must be thawed prior to use.
Some investigators have determined quantities of plasma miRNAs by comparing internal control miRNAs (Ng et al, 2009; Resnick et al, 2009).
Although some investigators have determined quantities of plasma miRNAs by comparing internal control miRNAs (Ng et al, 2009; Resnick et al, 2009), it remains controversial as to which miRNAs are suitable as internal control for plasma assay.
20, 22– 26 Most HIV viral load assays use quantitative reverse transcriptase polymerase chain reaction (PCR), which requires large quantities of plasma (100 600 μL) to transcribe RNA into DNA before amplification.
Although some investigators have determined quantities of plasma miRNAs by comparing internal control miRNAs (Ng et al, 2009), it remains controversial as to which miRNAs are suitable as internal controls for plasma assays.
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Plasma-derived vaccines had been produced in India since 1981, but concerns arose around the capacity to produce large quantities of plasma-derived vaccines, and about their safety since they are derived from human blood [ 14].
The North Carolina team announced a breakthrough in 1966 in the form of a concentrate for the blood factor, known as Factor VIII, that could be stored at room temperature, was far more potent than an equivalent quantity of plasma and could be manufactured at low cost.
When the coronal mass flares occur on the Sun, the quantity of plasma increases and solar wind shocks are generated.
The ion composition of plasma in the magnetosphere and the plasma sheet is one of important parameters in space physics, because it affects the Alfvén velocity that is a fundamental physical quantity of plasma.
As plasma contains small quantities of miRNA/RNA [ 31] and, typically, the quantity of plasma available is limited, we have previously evaluated techniques and kits to optimize isolation and yield [ 23, 32].
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