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After loading similar quantities of brain equivalent tissues on each lane, cleaved PrP, corresponding to the C2 fragment, was only abundant in mice with "C-BSE like" features.
However, the need of large quantities of brain tissue and elaborate protocols to isolate the fibrils have limited its use.
After confirmatory Western blot, insufficient quantities of brain tissue were available for Western blot discriminatory testing in 26 samples, which are shown in the Table as samples not analyzed.
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Every top show is like a comedy Los Alamos project - burning up vast quantities of brains, cunning, endurance and paranoia.
The clinical relevance of changes in quantity of brain amyloid over time, especially when disease-modifying treatments are available, should also be assessed.
However, the fact that PrPres generated in vitro was associated with approximately 10 times less infectivity than an equivalent quantity of brain-derived PrPSc casts doubt on the "protein-only" hypothesis of prion propagation and backs theories that suggest there are additional molecular species of infectious PrP or other agent-associated factors.
All spectra were processed offline using the LCModel software package [80] to determine the quantities of the brain metabolites N-acetylaspartate and N-acetylaspartylglutamate (collectively referred to as NAA), choline-containing compounds (referred to as Cho), myo-inositol (MI), and creatine-containing compounds (referred to as Cr).
The simultaneous communication between billions of neurons produces a large quantity of electric brain activity, which can be detected and measured through EEG technology.
Experimental controls for ic and ip transmissions were inoculated under the same experimental conditions using identical quantities of uninfected mouse brain material.
They said the experiment was going forward without evidence in monkeys that it could work and that it held the possibility of harm: viruses spreading in the brain, or gene-treated cells churning out huge quantities of proteins that inhibit brain cells from firing.
While the temporal resolution of microdialysis is limited, this technique can detect relatively fast changes in extracellular concentrations of various analytes with sampling times as low as 1 min. Our lab and others have extended its application to monitor the quantity and time course of brain concentrations of ethanol following systemic administration.
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