Sentence examples for quantities of amplification from inspiring English sources
Markers FCA149 and FCA097 had low quantities of amplification products.
In addition, to 30 cycles the quantity of amplification products in our PCR conditions is sufficiently visible in an ethidium bromide gel and keeps the lineal relationship to measured peak areas.
The size and the quantity of the amplification products were examined in agarose electrophoresis.
Although the quantity of the amplification product was higher in the absence (standard procedure) than in the presence of 60 mM TMAC (data not shown), the quality of MDA product, in terms of coverage and base composition, was improved in the optimized procedure where TMAC was added (Figs 2 and 3).
200ng of Total RNA was used as the starting quantity for amplification and labelling of all samples.
Potential benefits of using single-molecule sequencing are: the minimal quantities of input DNA required; elimination of amplification bias; asynchronous synthesis; fast turnaround times; and the capacity to investigate the characteristics of individual DNA molecules.
To avoid multiple rounds of amplification of small quantities of RNA, which would arise from less-abundant neuronal populations such as Parv-cre/TdT+, we chose to pool DRGs from cervical to lumbar regions (C1-L6).
Thus, they not only possess good specificity as that of hybridization reactions but also overcome the hurdle of amplification of smaller quantity targets as in, say, PCR based detection.
The results revealed that the extra washing step added to the RNA extraction process resulted in significantly improved RNA quantity and quality and improved success of amplification from paraffin-embedded specimens.
Although three rounds of amplification yielded enough quantity of RNA for microarray analysis (>20 μg) even in the case of 2 pg RNA, scatter plot analysis revealed that the qualities of the obtained results were quite different between the samples from 5 μg and 2 pg RNA.
The fold of amplification increases with smaller quantities of template RNA, but the absolute yield of aRNA decreases.
