Levels of GFP expression were semi-quantitatively quantified at 400 × magnification by scoring the percentage of GFP-stained cells per field in at least five fields per section.
Levels of expression were semi-quantitatively quantified by scoring the percentage of positive nuclei stained for each specific molecule per microscopic field in at least 5 fields per section by 2 blinded observers and, in rare cases of discrepancy, by an additional third independent observer.
The ion composition was first examined qualitatively to determine which ions were detectable, and then quantitatively to quantify their variation.
The amount of vitellogenin mRNA was quantified semi-quantitatively using actin as an active reference [11].The analysis documented that bees that on average had high numbers of ovarioles and high sucrose responsiveness (HH) also had higher relative levels of vitellogenin mRNA expression than bees with low numbers of ovarioles and low sucrose responsiveness (P = 0.0025; Fig. 2B).
Band intensities were quantified semi-quantitatively by three independent individuals using a four point (0 to 3) scoring method.
Patient No. reflects the number in Table 1; stainingining was quantified semi-quantitatively as described in the " Materials and methods" section.
Quantitatively, attempts to quantify risk have incorporated fuzzy unclear elements in risk analysis [39], and structural equation modeling has investigated marine accidents [40].
Quantitatively, the expected behavior (as quantified by the contaminant mass remaining in the aquifer) is statistically different between the positive and negative cases: on average, more contaminant mass persists when K and k are negatively correlated.
Our study aims to provide this annotation for 189 TCGA tumors, quantified by Nanostring and verified for EGFRvIII quantitatively by RT-PCR.
Expression of acetylated tubulin and spatacsin in neurons was quantified in situ by measuring the fluorescent intensity quantitatively [ 36) and ImageJ (http://rsbweb.nih.gov/ij/docs/guide/userguide-27.html)].
To determinate the turbidity quantitatively, the total intensity of scattering light has been measured and quantified as number of photons by adopting the single photon detection techniques (SPDT) which has the advantage of high sensitivity.
