To evaluate this homophilic interaction quantitatively, we prepared recombinant extracellular domains of the wild-type and mutated CADM1 proteins and examined their interactions by fluorescence correlation spectroscopy.
Morphine-6-O-glucuronide (7, M6G) was also synthesized similarly to that of compound 6 utilizing 3-O-acetylmorphine (2a) as the starting material which was prepared quantitatively by selective acetylation of morphine according to the method of Welsh [10].
The salt form of the compounds used for biological testing was prepared quantitatively by treating the acid in methanol with one equivalent of 0.5 M NaHCO3 solution.
Solutions of different concentrations were prepared by quantitatively diluting stock solutions using dry MeCN.
To correlate the photodetector signals with the algae species, a convolution algorithm was prepared to quantitatively compare photodetector signals (Fig. 3) with a set of test functions (Fig. 4 ).
To assess whether Y1043 contributes to catalysis, we prepared and quantitatively characterized the mutant Ire1KR32(Y1043F), which is predicted to disable any involvement of Y1043 in proton transfer while ensuring minimal structural perturbation to the protein compared to a potentially far more disruptive Y1043A mutation tested previously [ 6].
A solution of Cu II) solution (1000 µg/mL) was prepared by dissolving 2.5 g of CuSO4 quantitatively into a little distilled water contained in 1L volumetric flask and made up to the mark with distilled water.
However, the lamellarity of liposomes prepared by this method has not been evaluated quantitatively.
To explore the optimal substrates for this application, eight N-acyl derivatives of d-mannosamine were prepared, and their accessibility to NeuNAc aldolase was quantitatively investigated.
To quantitatively characterize loading, 2 × 106 T cells per sample were prepared for ICP-OES analysis by digesting the cells in three parts trace metal grade hydrochloric acid (Fisher Scientific, Pittsburgh, PA) and one part trace metal grade nitric acid (EMD Chemicals, Gibbstown, NJ) overnight.
