The degree of inflammatory infiltration in the liver was assessed semi-quantitatively as mild, moderate or severe, based on the number of cells and cell layers in the portal areas or between hepatic cords.
Valvular pathology and dysfunction was classified semi-quantitatively as mild, moderate, or severe.
In this study, neuropathological alterations (spongiform change, astrogliosis/neuronal loss, degree of various protein depositions) were semi-quantitatively (none, mild, moderate, severe) evaluated in different anatomical regions.
Then, for each candidate-biomarker, the mild stress induced candidate-biomarker responses were quantitatively correlated to mild stress induced robustness towards lethal stress per mild stress and lethal stress pair.
A high molecular weight polymer over 270,000 in the weight-average molecular weight was quantitatively obtained in mild conditions.
We identified both short- and long-term biomarkers of which their induction levels were quantitatively correlated to the mild stress induced enhanced robustness level of the cell.
Intensity of immunoreactivity was scored semi-quantitatively as absent, mild, moderate and strong on a 0 to 3 point scale.
The histological changes were estimated semi-quantitatively, as being mild, moderate, or marked by microscopic examination of histological sections.
Ten candidate-biomarkers were quantitatively measured before and after mild stress treatment for 2 to 60 min, namely, catalase activity, the proteins SigB, ClpC and ClpP, and the transcripts sigB, catA, catE, clpB, clpC, clpP.
Noninvasive enteropathogenic bacteria and Norovirus dominated quantitatively and were associated with mild to moderate symptoms.
