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Cell-type specific effects had already been reported by quantitative transcription analysis of a selected number of nuclear genes across two different human ρ° cell lines, their ρ+ counterparts, and fibroblasts from a patient affected by Kearns-Sayre syndrome (KSS) [10].
We developed procedures for the conversion of cDNA Differential Display data into quantitative transcription profiles.
Serial Analysis of Gene Expression [ 3] is a free, open-end, quantitative transcription profiling system.
According to the European Leukemia Net (ELN) assay, real-time quantitative transcription polymerase chain reaction (RQ-PCR) normalized to ABL gene was used to assess WT1 expression [ 6].
BioMark HD™ and OpenArray® are RT-qPCR based technologies which is considered as the gold standard for quantitative transcription analysis due to its high sensitivity, high reproducibility and large dynamic range [ 19– 219.
Proprietary high-end transcript profiling systems such as Massive Parallel Signature Sequencing [ 1] and GeneCalling [ 2] are excellent tools for genome-wide quantitative transcription analysis on any organism, but they are available to only a small fraction of researchers.
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Transcriptional changes were analyzed using microarrays and quantitative reverse transcription polymerase chain reaction.
quantitative reverse transcription polymerase chain reaction.
Quantitative reverse transcription PCR (qRT-PCR) has been routinely used to quantify gene expression level.
Validation of these 138 genes using quantitative reverse transcription PCR (qRT-PCR) confirmed 91 genes.
Fig. 4 Validation of the RNA-sequencing data by quantitative reverse transcription PCR (qRT-PCR).
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