Sentence examples for quantitative transcript from inspiring English sources

In this study, by combining the newest sequencing method, "Sequencing by oligonucleotide ligation and detection" (SOLiD™), with 5'SAGE, we have developed a 5'-end SOLiD technology (5'SOLiD) that can be used to identify transcriptional start sites and quantitative transcript levels in any cell type in a comprehensive fashion.

It may also provide a useful tool for identifying reference genes for quantitative transcript profiling.

To profile the expression pattern of S. aureus during nasal colonization we performed quantitative transcript analysis directly on nose swabs obtained from persistent carriers.

As quantitative transcript profiling by massively parallel sequencing is potentially affected by the accuracy of the mapping of short reads, we performed an in silico analysis to evaluate this.

qRT-PCR is the most common method of quantitative transcript analysis.

Quantitative transcript expression and genotype relationships can be investigated via linkage or association based methodologies.

Although the semi-quantitative transcript analysis shown in Figure 2D indicates that levels of aaxC expression are the same or lower than aaxA levels, we cannot rule out the possibility of multiple, unidentified promoters in this gene cluster.

The same pattern was seen in RNA from tissues sampled at the mid-secretory phase, and mRNA corresponding to the ERRβΔ10 form was never detected; although this method is only semi-quantitative, transcript abundance appeared higher in samples from the proliferative phase.

Quantitative reverse transcript PCR analysis was performed using the ABI PRISM7700 real-time PCR system (Applied Biosystems, Foster city, CA).

Besides the high throughput of RNA-seq overcomes the shortcoming of low redundancy of sequencing reads in EST data, which makes EST data not suitable for quantitative measuring transcript abundance [16], [18].

The results were consistent with findings obtained from quantitative phz transcript analysis by QRT-PCR, but were not consistent with the PCA quantity produced in wild type strain M18 or in the two mutants, suggesting a repression mechanism involved in the phzA1-G1 trandcript anotnot in the phzA2-G2 transcript exists at the post-transcriptional levels in strain M18.